IdeS | IdeZ蛋白酶/ IdeS | IdeZ protease
rIdeS | Idez蛋白酶, 质谱级
rIdeS | IdeZ Protease, MS Grade
订货号： HLS IDES001 | IDEZ001
说明： rIdeS | rIdeZ（免疫球蛋白IgG 特异性降解酶）是一种在大肠杆菌中过表达的工程重组蛋白酶。在铰链区下方的单个识别位点特异性切割，以产生同质的 F(ab´) 2 和 Fc 片段; IdeS 和 IdeZ 蛋白酶均可有效切割人 IgG1、IgG2、IgG3 和 IgG4、猴、绵羊、兔、人源化和嵌合 IgG 以及 Fc 融合蛋白。然而，只有 IdeZ 蛋白酶能切割小鼠 IgG2a 和 IgG3.
物理形态: 冻干粉
分子量: 37kDa.
溶解液： 加40ul超纯水溶解
储存条件：冻干粉末存储在-20℃冰箱，再溶解的酶储存在-20℃或- 80℃
保质期： 24月在-20℃
pH 值范围: 在PH6-8 时 具有最佳活性
适用范围： rIdeS | rIdeZ蛋白酶是IgG 特异性降解酶，产生同质的 F(ab´) 2 和 Fc 片段
酶切SOP:
- 取10ul 10ug/ul mAb 样品
- 加 5 µl 的 10X 酶切缓冲液（HLS PBS001)和 31ul 超纯水混合，总反应体积为50µl
- 加 4 µl 0.5ug/ul rIdeS 蛋白酶
- 在 37°C 下孵育 30 分钟
Part No. Name Size
HLS IDEZ001 rIdeZ 20 μg
HLS PBS001 digestion buffer 0.5 mL
Description: rideZ, MS grade, is an engineered recombinant protease overexpressed in Escherichia coli. It cleaves specifically at a single recognition site below the hinge region to produce homogenous F(ab’)2 and Fc fragments. Both IdeS and IdeZ proteases effectively cleave human IgG1, IgG2, IgG3, and IgG4, monkey, sheep, rabbit, humanized and chimeric IgG, and Fc fusion proteins. However, only the IdeZ protease cleaves mouse IgG2a and IgG3.
Physical Appearance: Lyophilized powder
Molecular Weight: 37 kDa
Resuspension Buffer (reference): 40 μL double-distilled water from user
Digestion Buffer: 10X 50 mM PBS, 150 mM NaCl (pH 6.6) solution
Storage Conditions: Powder at -20 °C
Shelf Life: 24 months at -20 °C
pH Range: Maximally active at pH 6-8
Application: rIdeS protease is an IgG-specific degradation enzyme to produce homogeneous F(ab’)2 and Fc fragments.
In-Solution Protein Digestion Protocol (Recommended):
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Mix 5 μL 10X enzyme digestion buffer (HLS PBS001) and double-distilled water.
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Add 10 μL 10 μg/μL mAb samples to the mixture.
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Add 4 μL 0.5 μg/μL rIdeS (HLS IDE001) to the mixture to total reaction volume of 50 μL.
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Incubate at 37 °C for 30 min.
Activity: 50 units/μg