BT表面活性剂/BT Surfactant
BT表面活性剂: BT Surfactant
BT 表面活性剂是一种新型的质谱兼容表面活性剂,用于溶液中蛋白质的提取、溶解和消化。可快速使蛋白质样品变性和溶解,并最大限度地提高蛋白质样品经蛋白酶切的回收率,重复性和消化效率。BT表面活性剂在蛋白质溶解和提取效率方面的性能与十二烷基磺酸钠 (SDS) 相似. 在45°C酸性条件下孵育30min可完全降解;在95°C高温加热3min也可以降解到0.01%以下。是最理想的蛋白变性剂和酶促反应活性剂。
产品用途:
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改进的蛋白质溶解和提取,特别是对于大的疏水蛋白质
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在高温下用于蛋白质完全变性并自身降解
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在低温下增强蛋白酶的酶切效率
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增强PTM多肽高回收率;重复性好
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解决蛋白不污染变性;多肽无需脱盐处理
产品特点:铵离子表面活性剂,质谱级试剂
Part No. Name Size
HLS BTS001C BT Surfactant 5 mg
Description: Protein denaturation and enzymolysis are essential experimental steps in analyzing peptides and glycans of biopharmaceutical antibodies and proteomics. The highly structural characteristics of proteins in their natural forms pose obstacles for enzymes to reach the cutting sites. In order to expose the proteins’ enzymatic cutting sites and make them effectively interact with the enzymes, proteins must undergo complete denaturation, reductive alkylation, and appropriate enzymatic reaction. Common denaturants, including urea, guanidine hydrochloride, and SDS, will carry out specific chemical modifications of the protein, such as carbamylation, which may cause the error in polypeptide identification. However, the BT Surfactant can effectively avoid many unfavorable post-translational modifications after protein denaturation.
Physical Appearance: Lyophilized powder
Molecular Weight: 393.28 Da
Resuspension Buffer (reference): Resuspend 5 mg BT Surfactant powder in 167 µL user’s buffer (pH 8.0) or double-distilled water to get 3% (w/v) solution.
Storage Conditions: Powder at - 20 °C, reconstituted solution at - 80 °C
Shelf Life: 24 months at -80 °C as solution; long-term effective at -20 °C as powder
pH Range: Maximally active for enzymatic reaction at pH 7-9; degrade and precipitate at pH 2-4. Temperature: < 1% BT Surfactant peak area after incubation at 37 °C for 4 hours; < 5% BT Surfactant peak are after incubation at 95 °C for 10 min.
Reference Procedure:
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Denature protein samples in 1-2% (w/v) BT Surfactant solution.
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Digest proteins with user’s protease in 0.1% (w/v) BT Surfactant solution.
BT57试剂, 质谱级
BT57 Reagent, MS Grade
说明: BT57试剂是快速的变性,还原和烷基化三合一试剂。在生物制药和蛋白质组学研究中,三维结构的天然蛋白质经过变性,还原和烷基化处理,脱盐后进行酶解反应。常规变性剂尿素,盐酸胍等对蛋白质变性后,会对蛋白质进行一定的化学修饰,如氨基甲酰化, 同时常规烷基化试剂需要避光。BT57试剂三合一试剂一步添加,快速完成变性,还原,烷基化蛋白处理。适用于蛋白组学,生物制药和单细胞样本研究。
物理形态: 冻干粉含1.5mg BT表面活性,TECP和CAA
分子量: 398.3Da.
溶解液: 52ul 超纯水溶解
存储条件: –20 °C冰箱冷冻.
保质期:24 个月 在 –20°C冰箱
pH值范围: PH7-9用于蛋白变性反应,PH2-4在溶液中会降解并沉淀
使用方法:
-10ul(50-100ug)蛋白样品添加10ul BT57试剂混匀,60°C干浴孵育20min或95°C干浴孵育10m
纯度:>99.9%。QE HF 超高分辨质谱ESI+ 离子检测杂质峰峰强度低于0.1%; ESI-离子检测杂质峰峰强度低于0.1%
Part No. Name Size
HLS BT57001 BT57 Reagent, Mass Spec Grade 1.5 mg
Description: BT57 is a fast, three-in-one reagent for denaturation, reduction, and alkylation, ideal for biopharmaceutical and proteomics research. In these fields, native protein structures are typically denatured, reduced, alkylated, desalted, and then prepared for enzymatic reactions. Conventional denaturants like urea and guanidine hydrochloride can chemically modify proteins (e.g., carbamylation), and traditional alkylating agents require light protection. BT57 addresses these issues, allowing for the rapid completion of denaturation, reduction, and alkylation in a single step. It is suitable for proteomics, biopharmaceuticals, and single-cell sample research.
Physical Appearance: Lyophilized powder
Molecular Weight: 393.28 Da
Resuspension Buffer: 52 μL double-distilled water dissolve.
Storage Conditions: Powder at - 20 °C, reconstituted solution at - 80 °C
Shelf Life: 24 months at -80 °C as a solution; long-term stable at -20 °C as powder
pH Range: pH 7-9 for protein denaturation reaction, will degrade and precipitate in solution at pH 2-4
Reference Protocol:
Add 10 µL BT57 to 10 µL (50 µg) protein sample, mix, and incubate at 60°C for 20 min or 95°C for 10 min.
Purity: > 99.9% based on BT Surfactant peak area, analyzed by Thermo QE HF mass spectrometry with positive ion mode ESI.
Digest Buffer, 质谱级
Digest Buffer, MS Grade
说明: Digest Buffer试剂是基于BT表面活性剂配制的缓冲液。在生物制药和蛋白质组学研究中,三维结构的天然蛋白质经过变性,还原和烷基化处理后,蛋白样品需要脱盐并进行酶解反应。在Digest Buffer中将蛋白水解为肽段,最大化提高酶切效率和回收率,然后加酸降解BT表面活性剂,多肽样品直接用于质谱分析,避免了冗繁的多肽脱盐步骤造成的修饰肽损失。适用于蛋白组学,生物制药和单细胞样本研究。
物理形态: 冻干粉含5mg BT表面活性和48mgABC
分子量: 398.3Da.
溶解液: 12ml 超纯水溶解, pH=8.0
存储条件: 4 °C冰箱储存.
保质期:6个月 在 4°C冰箱
pH值范围: PH7-9用于蛋白酶解实验,PH2-4在溶液中会降解并沉淀
使用方法:
-10ul(50ug)蛋白样品添加90ul Digest Buffer试剂混匀,加入1ug质谱级蛋白酶,37°C干浴孵育30min,然后加3ul甲酸45°C孵育20min
纯度:>99.9% QE HF 超高分辨质谱ESI+ 离子检测杂质峰峰强度低于0.1%; ESI-离子检测杂质峰峰强度低于0.1%
Part No. Name Size
HLS DIG001B Digest Buffer, Mass Spec Grade 5 mg
Description: Digest Buffer is a BT surfactant-based buffer optimized for proteomics and biopharmaceutical research. It facilitates protein denaturation, reduction, and alkylation, followed by enzymatic hydrolysis to convert proteins into peptides with high efficiency and recovery. The surfactant is then degraded by formic acid, allowing direct peptide analysis via mass spectrometry without the need for desalting, minimizing peptide loss. Ideal for proteomics, biopharmaceuticals, and single-cell research.
Physical Appearance: Lyophilized powder with 5 mg BT Surfactant and 48 mg ammonium bicarbonate
Molecular Weight: 393.28 Da
Resuspension Buffer: Dissolve in 12 mL MS Grade water
Storage Conditions: Powder at - 20 °C, reconstituted solution at 4 °C for 6 months
pH Range: pH 7-9 is used for proteolysis experiments, will degrade and precipitate in solution at pH 2-4
Reference Protocol:
Add 100 μL Digest Buffer to 10 μL (50 μg) protein sample and mix well. Add 1 μg MS-grade protease and incubate at 42°C for 40 minutes. Then, add 3 μL formic acid and incubate at 42°C for 20 minutes.
Purity: > 99.9% BT Surfactant peak area, analyzed by Thermo QE HF mass spectrometry coupled with positive ion mode ESI.