BT57试剂, 质谱级

BT57 Reagent, MS Grade

 

说明: BT57试剂是快速的变性，还原和烷基化三合一试剂。在生物制药和蛋白质组学研究中，三维结构的天然蛋白质经过变性，还原和烷基化处理，脱盐后进行酶解反应。常规变性剂尿素，盐酸胍等对蛋白质变性后，会对蛋白质进行一定的化学修饰，如氨基甲酰化, 同时常规烷基化试剂需要避光。BT57试剂三合一试剂一步添加,快速完成变性,还原,烷基化蛋白处理。适用于蛋白组学，生物制药和单细胞样本研究。

物理形态: 冻干粉含1.5mg BT表面活性，TECP和CAA

分子量: 398.3Da.

溶解液: 52ul 超纯水溶解

存储条件: –20 °C冰箱冷冻. 

保质期：24 个月 在 –20°C冰箱

pH值范围:  PH7-9用于蛋白变性反应，PH2-4在溶液中会降解并沉淀

 

使用方法:

-10ul(50-100ug)蛋白样品添加10ul BT57试剂混匀，60°C干浴孵育20min或95°C干浴孵育10m

 

纯度：>99.9%。QE HF 超高分辨质谱ESI+ 离子检测杂质峰峰强度低于0.1%； ESI-离子检测杂质峰峰强度低于0.1%

 

Part No.                       Name                                                          Size

HLS BT57001            BT57 Reagent, Mass Spec Grade        1.5 mg

 

Description: BT57 is a fast, three-in-one reagent for denaturation, reduction, and alkylation, ideal for biopharmaceutical and proteomics research. In these fields, native protein structures are typically denatured, reduced, alkylated, desalted, and then prepared for enzymatic reactions. Conventional denaturants like urea and guanidine hydrochloride can chemically modify proteins (e.g., carbamylation), and traditional alkylating agents require light protection. BT57 addresses these issues, allowing for the rapid completion of denaturation, reduction, and alkylation in a single step. It is suitable for proteomics, biopharmaceuticals, and single-cell sample research.

Physical Appearance: Lyophilized powder

Molecular Weight: 393.28 Da

Resuspension Buffer: 52 μL double-distilled water dissolve.

Storage Conditions: Powder at - 20 °C, reconstituted solution at - 80 °C

Shelf Life: 24 months at -80 °C as a solution; long-term stable at -20 °C as powder

pH Range: pH 7-9 for protein denaturation reaction, will degrade and precipitate in solution at pH 2-4

Reference Protocol: 

Add 10 µL BT57 to 10 µL (50 µg) protein sample, mix, and incubate at 60°C for 20 min or 95°C for 10 min.

Purity: > 99.9% based on BT Surfactant peak area, analyzed by Thermo QE HF mass spectrometry with positive ion mode ESI.