BT表面活性剂: BT Surfactant

 

BT 表面活性剂是一种新型的质谱兼容表面活性剂，用于溶液中蛋白质的提取、溶解和消化。可快速使蛋白质样品变性和溶解，并最大限度地提高蛋白质样品经蛋白酶切的回收率，重复性和消化效率。BT表面活性剂在蛋白质溶解和提取效率方面的性能与十二烷基磺酸钠 (SDS) 相似. 在45°C酸性条件下孵育30min可完全降解；在95°C高温加热3min也可以降解到0.01%以下。是最理想的蛋白变性剂和酶促反应活性剂。

 

产品用途：

• 改进的蛋白质溶解和提取，特别是对于大的疏水蛋白质

• 在高温下用于蛋白质完全变性并自身降解

• 在低温下增强蛋白酶的酶切效率

• 增强PTM多肽高回收率；重复性好

• 解决蛋白不污染变性；多肽无需脱盐处理

 

产品特点：铵离子表面活性剂，质谱级试剂

 

Part No.                                  Name                                         Size

HLS BTS001C                       BT Surfactant                          5 mg

 

Description: Protein denaturation and enzymolysis are essential experimental steps in analyzing peptides and glycans of biopharmaceutical antibodies and proteomics. The highly structural characteristics of proteins in their natural forms pose obstacles for enzymes to reach the cutting sites. In order to expose the proteins’ enzymatic cutting sites and make them effectively interact with the enzymes, proteins must undergo complete denaturation, reductive alkylation, and appropriate enzymatic reaction. Common denaturants, including urea, guanidine hydrochloride, and SDS, will carry out specific chemical modifications of the protein, such as carbamylation, which may cause the error in polypeptide identification. However, the BT Surfactant can effectively avoid many unfavorable post-translational modifications after protein denaturation.

Physical Appearance: Lyophilized powder

Molecular Weight: 393.28 Da

Resuspension Buffer (reference): Resuspend 5 mg BT Surfactant powder in 167 µL user’s buffer (pH 8.0) or double-distilled water to get 3% (w/v) solution.

Storage Conditions: Powder at - 20 °C, reconstituted solution at - 80 °C

Shelf Life: 24 months at -80 °C as solution; long-term effective at -20 °C as powder

pH Range: Maximally active for enzymatic reaction at pH 7-9; degrade and precipitate at pH 2-4. Temperature: < 1% BT Surfactant peak area after incubation at 37 °C for 4 hours; < 5% BT Surfactant peak are after incubation at 95 °C for 10 min.

Reference Procedure:

1. Denature protein samples in 1-2% (w/v) BT Surfactant solution.

1. Digest proteins with user’s protease in 0.1% (w/v) BT Surfactant solution.