Digest Buffer, 质谱级

Digest Buffer, MS Grade

 

说明: Digest Buffer试剂是基于BT表面活性剂配制的缓冲液。在生物制药和蛋白质组学研究中，三维结构的天然蛋白质经过变性，还原和烷基化处理后，蛋白样品需要脱盐并进行酶解反应。在Digest Buffer中将蛋白水解为肽段，最大化提高酶切效率和回收率，然后加酸降解BT表面活性剂，多肽样品直接用于质谱分析，避免了冗繁的多肽脱盐步骤造成的修饰肽损失。适用于蛋白组学，生物制药和单细胞样本研究。

物理形态: 冻干粉含5mg BT表面活性和48mgABC

分子量: 398.3Da.

溶解液: 12ml 超纯水溶解, pH=8.0

存储条件: 4 °C冰箱储存. 

保质期：6个月 在 4°C冰箱

pH值范围:  PH7-9用于蛋白酶解实验，PH2-4在溶液中会降解并沉淀

 

使用方法:

-10ul(50ug)蛋白样品添加90ul Digest Buffer试剂混匀，加入1ug质谱级蛋白酶，37°C干浴孵育30min，然后加3ul甲酸45°C孵育20min

纯度：>99.9%  QE HF 超高分辨质谱ESI+ 离子检测杂质峰峰强度低于0.1%； ESI-离子检测杂质峰峰强度低于0.1%

Part No.                   Name                                                        Size

HLS DIG001B        Digest Buffer, Mass Spec Grade        5 mg

 

Description: Digest Buffer is a BT surfactant-based buffer optimized for proteomics and biopharmaceutical research. It facilitates protein denaturation, reduction, and alkylation, followed by enzymatic hydrolysis to convert proteins into peptides with high efficiency and recovery. The surfactant is then degraded by formic acid, allowing direct peptide analysis via mass spectrometry without the need for desalting, minimizing peptide loss. Ideal for proteomics, biopharmaceuticals, and single-cell research.

Physical Appearance: Lyophilized powder with 5 mg BT Surfactant and 48 mg ammonium bicarbonate

Molecular Weight: 393.28 Da

Resuspension Buffer: Dissolve in 12 mL MS Grade water

Storage Conditions: Powder at - 20 °C, reconstituted solution at 4 °C for 6 months

pH Range: pH 7-9 is used for proteolysis experiments, will degrade and precipitate in solution at pH 2-4

Reference Protocol: 

Add 100 μL Digest Buffer to 10 μL (50 μg) protein sample and mix well. Add 1 μg MS-grade protease and incubate at 42°C for 40 minutes. Then, add 3 μL formic acid and incubate at 42°C for 20 minutes.

Purity: > 99.9% BT Surfactant peak area, analyzed by Thermo QE HF mass spectrometry coupled with positive ion mode ESI.