

糖苷酶 PNGase F/ Endoproteinase PNGase F
PNGase F糖苷酶, 质谱级
PNGase F Glycodase, MS Grade
订货号: HLS PNG001C
说明: 质谱级PNGase F 糖苷酶特异性水解复杂的糖蛋白的寡糖和高甘露糖链接的天冬酰胺,酶切掉天冬氨酸脱氨基的天冬酰胺,保留低聚糖蛋白完好无损。但是不会去除低聚糖含有的α(1→3)核心岩藻糖。
物理形态: 20mM Tris-HCl , 50mM NaCl and 5mM EDTA 溶液
分子量: 36kDa.
浓度:10unit/ul
储存条件:存储在4-10℃冰箱,禁止-20℃以下低温冷冻
保质期:12个月,在4℃条件下
pH 范围: PH6.0–10.0 有活性, PH=8.6具有最佳活性
适用范围:特异性酶切糖蛋白的 N端寡糖
溶液酶解SOP:
1. 在 50mM Ammonium Bicarbonate (pH 7.8)溶液中加入20ug糖蛋白,调整体积至 18µl.
2. 加 2µl of 复溶的PNGase F
3. 37°C 保温 2–18 小时
活性: 40000unit/min/mg;
单位定义:PNGase F糖苷酶每分钟酶切1.0 umole Ribonuclease B的量,反应条件:37℃,PH=7.5
Part No. Name Size /pkg
HLS PNG001C rPNGase F, Mass Spec Grade 50 μL
Description: PNGase F (Peptide-N-glycosidase F) is a protease for deglycosylation of glycoproteins, and is produced by recombinant protein expression. The enzyme hydrolyze the N-terminal of the asparagine (Asn) residue side chain to release asparagine-linked oligosaccharides from glycoproteins and glycopeptides. The oligosaccharides can be high mannose, hybrid, or complex type.
Molecular Weight: 35 kDa
Composition: rPNGase F in 20 mM Tris-HCl (pH 7.5 at 25 °C), 50 mM NaCl, and 5 mM EDTA solution
Concentration: 10 units/μL
Storage Conditions: Store at 2–8 °C.
Shelf life: 12 months at 2–8 °C
Stability: rPNGase F is maximally active in the pH range of 6-10, best at pH 8.6 .
In-Solution Protein Digestion Protocol:
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For maximum activity, resuspend the user’s samples (eg. glycoprotein) with 50 mM ammonium bicarbonate buffer (pH 7.8).
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Dissolve 20 μg samples in 50 mM ammonium bicarbonate (pH 7.8) to a final volume of 18 μL.
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Add 2 μL rPNGase F to the solution in step 2.. Mix well and incubate at 37 °C for 30 min.
Activity: 40,000 units/min/mg

rEndo S2糖苷酶 / Glycosidase rEndo S2
rEndo S2糖苷酶, 质谱级
rEndo S2 Glycosidase, MS Grade
订货号: HLS ENDOS2
说明: rEndo S2 糖苷酶来源于化脓性链球菌,是一种IgG特异性内切糖苷酶,该酶在大肠杆菌中表达。该酶可特异性水解天然 IgG Fc GlcNAc之后的N-糖链,从而使 GlcNAc 保留或保留附着的岩藻糖残基。该酶可水解所有人类 IgG 亚类和许多其他物种的 IgG 的 Fc N-糖。用于研究抗体无岩藻糖基化水平;去糖基化抗体免疫亲和特性;N-糖链上核心岩藻糖缺乏的ADCC效应或在抗体Fc核心GlcNAcs的定点偶联ADC药物以及血清中IgG的岩藻糖化水平。
物理形态: 含10mM PB (pH 7.4 at 25°C), 150mM NaCl 冻干粉
分子量: 92.5kDa.
内容物: 20ug
存储条件: –20 °C冰箱冷冻.
保质期:24 个月 在 –20°C冰箱
溶解液: 加80ul水复溶
pH值范围: rEndo S2糖苷酶在pH7.4活性最佳
酶切方法:
-适用酶切缓冲溶液:20mmol PB or Tris 缓冲液;, pH值在7-8范围内
-IgG蛋白样品,按照200:1蛋白和蛋白酶的量使用,酶切反应蛋白浓度控制在1ug/ul, 37°C干浴孵育30min以上
Part No. Name Size /pkg
HLS ENDOS2 r Endo S2, Mass Spec Grade 20 μg