糖苷酶 PNGase F/ Endoproteinase PNGase F
PNGase F糖苷酶, 质谱级
订货号: HLS PNG001C
说明: 质谱级PNGase F 糖苷酶特异性水解复杂的糖蛋白的寡糖和高甘露糖链接的天冬酰胺,酶切掉天冬氨酸脱氨基的天冬酰胺,保留低聚糖蛋白完好无损。但是不会去除低聚糖含有的α(1→3)核心岩藻糖。
物理形态: 20mM Tris-HCl , 50mM NaCl and 5mM EDTA 溶液
分子量: 36kDa.
浓度:10unit/ul
储存条件:存储在4-10℃冰箱,禁止-20℃以下低温冷冻
保质期:12个月,在4℃条件下
pH 范围: PH6.0–10.0 有活性, PH=8.6具有最佳活性
适用范围:特异性酶切糖蛋白的 N端寡糖
溶液酶解SOP:
1. 在 50mM Ammonium Bicarbonate (pH 7.8)溶液中加入20ug糖蛋白,调整体积至 18µl.
2. 加 2µl of 复溶的PNGase F
3. 37°C 保温 2–18 小时
活性: 40000unit/min/mg;
单位定义:PNGase F糖苷酶每分钟酶切1.0 umole Ribonuclease B的量,反应条件:37℃,PH=7.5
PNGase F, Mass Spec Grade
Part No. Name Size/pkg
HLS PNG001C PNGase F, Mass Spec Grade 100ul
Description: Glycosidase PNGase F, Mass Spec Grade, (Peptide N-Glycosidase F) cleaves asparagine-linked high mannose as well as hybrid and complex oligosaccharides from glycoproteins. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact . PNGase F will not remove oligosaccharides containing α(1→3)-linked core fucose.
Physical form: PNGase F is supplied as a liquid in 20mM Tris-HCl , 50mM NaCl and 5mM EDTA at a concentration of 10,000u/ml
Molecular Weight: 36kDa.
Storage Conditions: Store at 4°C-10°C, not freezed over -20°C
Shelf life:12 months at 4℃
Stability: PNGase F is maximally active in the pH range of 8.6 .
scope:PNGase F can effectively remove all N-glycans from both conserved and non-conserved glyco-sylation sites.
In-Solution Protein Digestion:
1. Add up to 20µg of glycoprotein in 50mM Ammonium Bicarbonate (pH 7.8) to a final volume of 18µl.
2. Add 2µl of recombinant PNGase F
3. Incubate at 37°C for 2–18 hours
Activity: 40000unit/min/mg;
Unit Definition:One unit will catalyze the release of N-linked oligosaccharides from one nanomole of denatured Ribonuclease B in 1 minute at pH 7.5 at 37 °C. One unit of PNGase F activity is equal to 1 IUB milliunit.