PNGase F糖苷酶, 质谱级

PNGase F Glycodase, MS Grade

 

订货号： HLS PNG001C

说明: 质谱级PNGase F 糖苷酶特异性水解复杂的糖蛋白的寡糖和高甘露糖链接的天冬酰胺，酶切掉天冬氨酸脱氨基的天冬酰胺,保留低聚糖蛋白完好无损。但是不会去除低聚糖含有的α(1→3)核心岩藻糖。

 

物理形态:  20mM Tris-HCl , 50mM NaCl and 5mM EDTA 溶液

分子量: 36kDa.

浓度：10unit/ul

储存条件：存储在4-10℃冰箱，禁止－20℃以下低温冷冻 

保质期：12个月，在4℃条件下

pH 范围:  PH6.0–10.0 有活性， PH=8.6具有最佳活性

适用范围：特异性酶切糖蛋白的 N端寡糖

溶液酶解SOP:

1. 在 50mM Ammonium Bicarbonate (pH 7.8)溶液中加入20ug糖蛋白，调整体积至 18µl.

2. 加 2µl of 复溶的PNGase F

3.  37°C 保温 2–18 小时

活性:   40000unit/min/mg；

单位定义：PNGase F糖苷酶每分钟酶切1.0 umole Ribonuclease B的量，反应条件：37℃，PH=7.5

 

Part No.                     Name                                                Size /pkg

HLS PNG001C         rPNGase F, Mass Spec Grade     50 μL

 

Description: PNGase F (Peptide-N-glycosidase F) is a protease for deglycosylation of glycoproteins, and is produced by recombinant protein expression. The enzyme hydrolyze the N-terminal of the asparagine (Asn) residue side chain to release asparagine-linked oligosaccharides from glycoproteins and glycopeptides. The oligosaccharides can be high mannose, hybrid, or complex type.

Molecular Weight: 35 kDa

Composition: rPNGase F in 20 mM Tris-HCl (pH 7.5 at 25 °C), 50 mM NaCl, and 5 mM EDTA solution

Concentration: 10 units/μL

Storage Conditions: Store at 2–8 °C. 

Shelf life: 12 months at 2–8 °C

Stability: rPNGase F is maximally active in the pH range of 6-10, best at pH 8.6 .

In-Solution Protein Digestion Protocol:

1. For maximum activity, resuspend the user’s samples (eg. glycoprotein) with 50 mM ammonium bicarbonate buffer (pH 7.8).

2. Dissolve 20 μg samples in 50 mM ammonium bicarbonate (pH 7.8) to a final volume of 18 μL.

3. Add 2 μL rPNGase F to the solution in step 2.. Mix well and incubate at 37 °C for 30 min.

Activity: 40,000 units/min/mg

 

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