糖苷酶 PNGase F/ Endoproteinase PNGase F

PNGase F糖苷酶, 质谱级

订货号： HLS PNG001C

说明: 质谱级PNGase F 糖苷酶特异性水解复杂的糖蛋白的寡糖和高甘露糖链接的天冬酰胺，酶切掉天冬氨酸脱氨基的天冬酰胺,保留低聚糖蛋白完好无损。但是不会去除低聚糖含有的α(1→3)核心岩藻糖。

物理形态:  20mM Tris-HCl , 50mM NaCl and 5mM EDTA 溶液

分子量: 36kDa.

浓度：10unit/ul

储存条件：存储在4-10℃冰箱，禁止－20℃以下低温冷冻 

保质期：12个月，在4℃条件下

pH 范围:  PH6.0–10.0 有活性， PH=8.6具有最佳活性

适用范围：特异性酶切糖蛋白的 N端寡糖

溶液酶解SOP:

1. 在 50mM Ammonium Bicarbonate (pH 7.8)溶液中加入20ug糖蛋白，调整体积至 18µl.

2. 加 2µl of 复溶的PNGase F

3.  37°C 保温 2–18 小时

活性:   40000unit/min/mg；

单位定义：PNGase F糖苷酶每分钟酶切1.0 umole Ribonuclease B的量，反应条件：37℃，PH=7.5

PNGase F, Mass Spec Grade

Part No.                      Name                                            Size/pkg  

HLS PNG001C           PNGase F, Mass Spec Grade            100ul

Description: Glycosidase PNGase F, Mass Spec Grade, (Peptide N-Glycosidase F) cleaves asparagine-linked high mannose as well as hybrid and complex oligosaccharides from glycoproteins. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact . PNGase F will not remove oligosaccharides containing α(1→3)-linked core fucose.

Physical form: PNGase F is supplied as a liquid in 20mM Tris-HCl , 50mM NaCl and 5mM EDTA at a concentration of 10,000u/ml

Molecular Weight: 36kDa.

Storage Conditions: Store at 4°C-10°C, not freezed over -20°C

Shelf life：12 months at 4℃

Stability: PNGase F is maximally active in the pH range of 8.6 .

scope：PNGase F can effectively remove all N-glycans from both conserved and non-conserved  glyco-sylation sites.

In-Solution Protein Digestion:

1. Add up to 20µg of glycoprotein in 50mM Ammonium Bicarbonate (pH 7.8) to a final volume of 18µl.

2. Add 2µl of recombinant PNGase F

3. Incubate at 37°C for 2–18 hours

Activity:  40000unit/min/mg;

Unit Definition:One unit will catalyze the release of N-linked oligosaccharides from one nanomole of denatured Ribonuclease B in 1 minute at pH 7.5 at 37 °C. One  unit of PNGase F activity is equal to 1 IUB milliunit.